KAIPTC-LMS

De Novo Antibody Sequencing

Using several trypsins to initially dissolve the antibody samples into overlapping peptides.

Optimization of fragmentation after LC-MS/MS digestion.

Characterizing the overlapped peptides by de novo peptide sequencing.

Automated assembly of the antibody sequence using the characterized peptides.

Detailed data analysis using state-of-the-art bioinformatics methods to accurately determine the amino acid sequence of the digested peptide.