Antibody Production and QC
The Introduction and Application of Prokaryotic Expression
Since the first glance at the incredible protein world of all beautiful organisms, the respectful scientists cracked the mysterious amino acid codes. Further diligent efforts have greatly reformed the whole scientific thinking by the powerful genetic engineering. With tremendous ignited passion on the basic research of numerous recombinant polypeptides and requirements of therapeutic proteins and antibodies, a number of the most talented scientists developed several prokaryotic organisms for heterologous expression and accumulation with sophisticated mechanisms. As a star microorganism, Escherichia coli (E. coli) was the first prokaryotic host developed for the genetic engineering and heterologous expression. Since this little bacterium is extremely easy to manipulate with genetic techniques and ferment with flexible scales, it is widely applied for numerous research and the most fermentation of industrial enzymes and pharmaceutical protein drugs. Noticeable, a Gram-positive bacterium (Bacillus subtilis) has been emerging as a valuable alternative prokaryotic host with excellent performance. Actually, it has become the popular bacterial champion for more and more applications for heterologous protein expression among several widely used prokaryotic organisms to produce enzymes, other functional polypeptides, and therapeutic antibodies.
Features of OmicsAb™ Prokaryotic Antibody Production
- Successfully expressed hundreds of targets with an excellent success rate of soluble expression up to 95 %.
- Harbor a magic library composed of various sets of expression vectors, signal peptides, modifications, tags (6-8 His, GST, SUMO, Trx, NusA, etc.), and every detailed element, such as selection marker and promoter.
- Possess various prokaryotic host strains suitable for standard expression, enhanced expression, toxic expression, and other specific expression with particular or combined requirement.
- Offer production with various scales from small-scale (several micrograms) up to gram production.
- One-stop service: start from the original design and optimization, all the way down to the target purification and data analysis report.